BROWSE

Related Scientist

Researcher

분자 분광학 및 동력학 연구단
분자 분광학 및 동력학 연구단
more info

Two distinct actin waves correlated with turns-and-runs of crawling microglia

Cited 0 time in webofscience Cited 0 time in scopus
22 Viewed 6 Downloaded
Title
Two distinct actin waves correlated with turns-and-runs of crawling microglia
Author(s)
Yang, Taeseok Daniel; Park, Kwanjun; Jin-Sung Park; Lee, Jang-Hoon; Choi, Eunpyo; Lee, Jonghwan; Wonshik Choi; Choi, Youngwoon; Lee, Kyung J.
Publication Date
2019-08
Journal
PLOS ONE, v.14, no.8, pp.e0220810 -
Publisher
PUBLIC LIBRARY SCIENCE
Abstract
© 2019 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Freely crawling cells are often viewed as randomly moving Brownian particles but they generally exhibit some directional persistence. This property is often related to their zigzag motile behaviors that can be described as a noisy but temporally structured sequence of “runs” and “turns.” However, its underlying biophysical mechanism is largely unexplored. Here, we carefully investigate the collective actin wave dynamics associated with the zigzag-crawling movements of microglia (as primary brain immune cells) employing a number of different quantitative imaging modalities including synthetic aperture microscopy and optical diffraction tomography, as well as conventional fluorescence imaging and scanning electron microscopy. Interestingly, we find that microglia exhibit two distinct types of actin waves working at two quite different time scales and locations, and they seem to serve different purposes. One type of actin waves is fast “peripheral ruffles” arising spontaneously with an oscillating period of about 6 seconds at some portion of the leading edge of crawling microglia, where the vigorously biased peripheral ruffles seem to set the direction of a new turn (in 2-D free space). When the cell turning events are inhibited with a physical confinement (in 1-D track), the peripheral ruffles still exist at the leading edge with no bias but showing phase coherence in the cell crawling direction. The other type is “dorsal actin waves” which also exhibits an oscillatory behavior but with a much longer period of around 2 minutes compared to the fast “peripheral ruffles”. Dorsal actin waves (whether the cell turning events are inhibited or not) initiate in the lamellipodium just behind the leading edge, travelling down toward the core region of the cell and disappear. Such dorsal wave propagations seem to be correlated with migration of the cell. Thus, we may view the dorsal actin waves are connected with the “run” stage of cell body, whereas the fast ruffles at the leading front are involved in the “turn” stage
URI
https://pr.ibs.re.kr/handle/8788114/6291
ISSN
1932-6203
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
Files in This Item:
Two distinct actin waves correlated_양태석, 박진성, 최원식(공동연구).pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse