BROWSE

Related Scientist

Researcher

나노의학 연구단
나노의학 연구단
more info

Small, Clickable, and Monovalent Magnetofluorescent Nanoparticles Enable Mechanogenetic Regulation of Receptors in a Crowded Live-Cell Microenvironment

Cited 0 time in webofscience Cited 0 time in scopus
17 Viewed 0 Downloaded
Title
Small, Clickable, and Monovalent Magnetofluorescent Nanoparticles Enable Mechanogenetic Regulation of Receptors in a Crowded Live-Cell Microenvironment
Author(s)
Minsuk Kwak; Wonji Gu; Heekyung Jeong; Hyunjung Lee; Jung-uk Lee; Minji An; Yong Ho Kim; Jae-Hyun Lee; Jinwoo Cheon; Young-wook Jun
Publication Date
2019-06
Journal
NANO LETTERS, v.19, no.6, pp.3761 - 3769
Publisher
AMER CHEMICAL SOC
Abstract
Multifunctional magnetic nanoparticles have shown great promise as next-generation imaging and perturbation probes for deciphering molecular and cellular processes. As a consequence of multicomponent integration into a single nanosystem, pre-existing nanoprobes are typically large and show limited access to biological targets present in a crowded microenvironment. Here, we apply organic-phase surface PEGylation, click chemistry, and charge-based valency discrimination principles to develop compact, modular, and monovalent magnetofluorescent nanoparticles (MFNs). We show that MFNs exhibit highly efficient labeling to target receptors present in cells with a dense and thick glycocalyx layer. We use these MFNs to interrogate the E-cadherin-mediated adherens junction formation and F-actin polymerization in a three-dimensional space, demonstrating the utility as modular and versatile mechanogenetic probes in the most demanding single-cell perturbation applications. © 2019 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/6229
ISSN
1530-6984
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > Journal Papers
Files in This Item:
201906_Small, Clickable, and Monovalent Magnetofluorescent Nanoparticles Enable Mechanogenetic Regulation of Receptors in a Crowded Live-Cell Microenvironment_Jinwoo Cheon Young-wook Jun.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse