Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm

Cited 0 time in webofscience Cited 0 time in scopus
46 Viewed 13 Downloaded
Title
Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm
Author(s)
Moonseok Kim; Yonghyeon Jo; Jin Hee Hong; Kim, Suhyun; Seokchan Yoon; Kyung-Deok Song; Kang, Sungsam; Lee, Byunghak; Kim, Guang Hoon; Park, Hae-Chul; Wonshik Choi
Publication Date
2019-07
Journal
NATURE COMMUNICATIONS, v.10, no.1, pp.3152 -
Publisher
NATURE PUBLISHING GROUP
Abstract
© 2019, The Author(s).Label-free in vivo imaging is crucial for elucidating the underlying mechanisms of many important biological systems in their most native states. However, the applicability of existing modalities has been limited to either superficial layers or early developmental stages due to tissue turbidity. Here, we report a synchronous angular scanning microscope for the rapid interferometric recording of the time-gated reflection matrix, which is a unique matrix characterizing full light-specimen interaction. By applying single scattering accumulation algorithm to the recorded matrix, we removed both high-order sample-induced aberrations and multiple scattering noise with the effective aberration correction speed of 10,000 modes/s. We demonstrated in vivo imaging of whole neural network throughout the hindbrain of the larval zebrafish at a matured stage where physical dissection used to be required for conventional imaging. Our method will expand the scope of applications for optical imaging, where fully non-invasive interrogation of living specimens is critical
URI
https://pr.ibs.re.kr/handle/8788114/6132
ISSN
2041-1723
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
Files in This Item:
Label-free neuroimaging in vivo using synchronous_김문석(최원식).pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse