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Development of a Universal Fluorescent Probe for Gram-Positive Bacteria

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Title: Development of a Universal Fluorescent Probe for Gram-Positive Bacteria

Author(s): Haw-Young Kwon; Xiao Liu; Eun Gyeong Choi; Jung Yeol Lee; So-Young Choi; Jun-Young Kim; Lu Wang; Sung-Jin Park; Beomsue Kim; Yong-An Lee; Jong Jin Kim; Nam Young Kang; Young Tae Chang

Subject: bioimaging, ; boronic acid, ; fluorescent probes, ; Gram-positive bacteria, ; structure–activity relationships

Journal: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.58, no.25, pp.8426 - 8431

Abstract: © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram-positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram-positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application