Biologically-active unilamellar vesicles from red blood cells

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Title
Biologically-active unilamellar vesicles from red blood cells
Author(s)
Hyun-Sook Jang; Yoon-Kyoung Cho; Steve Granick
Publication Date
2019-04
Journal
BIOMATERIALS SCIENCE, v.7, no.4, pp.1393 - 1398
Publisher
ROYAL SOC CHEMISTRY
Abstract
We demonstrate a method to prepare giant unilamellar vesicles (GUVs) with biologically-active protein activity, by mixing erythrocyte (red blood cell) membrane extract with phospholipids and growing their mixture in a porous hydrogel matrix. This presents a pathway to retain protein biological activity without prior isolation and purification of the protein, though only the activity of the membrane protein GLUT1 is investigated to date. Using the cascade enzymatic reaction glucose oxidase and horseradish peroxidase to assay glucose concentration specifically within the GUV interior, we show that glucose is internalized by GLUT1 whereas adding cytochalasin B, a GLUT1 inhibitor, blocks glucose transport. The method presented here operates at biological ionic strength and is both simple and potentially generalizable. © 2019 The Royal Society of Chemistry
URI
https://pr.ibs.re.kr/handle/8788114/5987
ISSN
2047-4830
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
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