Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions

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Title
Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
Author(s)
Jung, H; Seong-Wook Kim; Minsoo Kim; Hong, J; Yu, D; Ji Hye Kim; Yunju Lee; Kim, S; Doyeon Woo; Hee-Sup Shin; Byung Ouk Park; Won Do Heo
Publication Date
2019-01
Journal
NATURE COMMUNICATIONS, v.10, no., pp.314 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Spatiotemporal control of gene expression or labeling is a valuable strategy for identifying functions of genes within complex neural circuits. Here, we develop a highly light-sensitive and efficient photoactivatable Flp recombinase (PA-Flp) that is suitable for genetic manipulation in vivo. The highly light-sensitive property of PA-Flp is ideal for activation in deep mouse brain regions by illumination with a noninvasive light-emitting diode. In addition, PA-Flp can be extended to the Cre-lox system through a viral vector as Flp-dependent Cre expression platform, thereby activating both Flp and Cre. Finally, we demonstrate that PA-Flp-dependent, Cre-mediated Ca(v)3.1 silencing in the medial septum increases object-exploration behavior in mice. Thus, PA-Flp is a noninvasive, highly efficient, and easy-to-use optogenetic module that offers a side-effect-free and expandable genetic manipulation tool for neuroscience research. © The Author(s) 2019.
URI
https://pr.ibs.re.kr/handle/8788114/5756
ISSN
2041-1723
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
201901 Nature Communications.pdfDownload

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