Intensiometric biosensors visualize the activity of multiple small GTPases in vivo

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Title
Intensiometric biosensors visualize the activity of multiple small GTPases in vivo
Author(s)
Kim J.; Sangkyu Lee; Jung K.; Oh W.C.; Nury Kim; Son S.; Jo Y.J.; Kwon H.-B.; Won Do Heo
Publication Date
2019-01
Journal
NATURE COMMUNICATIONS, v.10, no.1, pp.211 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Ras and Rho small GTPases are critical for numerous cellular processes including cell division, migration, and intercellular communication. Despite extensive efforts to visualize the spatiotemporal activity of these proteins, achieving the sensitivity and dynamic range necessary for in vivo application has been challenging. Here, we present highly sensitive intensiometric small GTPase biosensors visualizing the activity of multiple small GTPases in single cells in vivo. Red-shifted sensors combined with blue light-controllable optogenetic modules achieved simultaneous monitoring and manipulation of protein activities in a highly spatiotemporal manner. Our biosensors revealed spatial dynamics of Cdc42 and Ras activities upon structural plasticity of single dendritic spines, as well as a broad range of subcellular Ras activities in the brains of freely behaving mice. Thus, these intensiometric small GTPase sensors enable the spatiotemporal dissection of complex protein signaling networks in live animals. © 2019, The Author(s)
URI
https://pr.ibs.re.kr/handle/8788114/5747
ISSN
2041-1723
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
20190114 Nature Communications.pdfDownload

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