PKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs

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Title
PKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs
Author(s)
Yoosik Kim; Joha Park; Sujin Kim; MinA Kim; Myeong-Gyun Kang; Chulhwan Kwak; Minjeong Kang; Baekgyu Kim; Hyun-Woo Rhee; V. Narry Kim
Publication Date
2018-09
Journal
MOLECULAR CELL, v.71, no.6, pp.1051 - 1.06E+09
Publisher
CELL PRESS
Abstract
Protein kinase RNA-activated (PKR) induces immune response by sensing viral double-stranded RNAs (dsRNAs). However, growing evidence suggests that PKR can also be activated by endogenously expressed dsRNAs. Here, we capture these dsRNAs by formaldehyde-mediated crosslinking and immunoprecipitation sequencing and find that various noncoding RNAs interact with PKR. Surprisingly, the majority of the PKR-interacting RNA repertoire is occupied by mitochondrial RNAs (mtRNAs). MtRNAs can form intermolecular dsRNAs owing to bidirectional transcription of the mitochondrial genome and regulate PKR and eIF2�� phosphorylation to control cell signaling and translation. Moreover, PKR activation by mtRNAs is counteracted by PKR phosphatases, disruption of which causes apoptosis from PKR overactivation even in uninfected cells. Our work unveils dynamic regulation of PKR even without infection and establishes PKR as a sensor for nuclear and mitochondrial signaling cues in regulating cellular metabolism. By employing formaldehyde crosslinking, Kim et al. provided genome-wide analysis of cellular dsRNAs that can interact with immune response protein PKR. They identify numerous noncoding RNAs that bind to PKR and reveal mitochondrial RNAs, which exist as intermolecular dsRNAs, that can regulate PKR phosphorylation and downstream signaling, especially under stress. ? 2018 Elsevier Inc
URI
https://pr.ibs.re.kr/handle/8788114/5433
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
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