BROWSE

Related Scientist

rna's photo.

rna
rna연구단
more info

ITEM VIEW & DOWNLOAD

PKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs

DC Field Value Language
dc.contributor.authorYoosik Kim-
dc.contributor.authorJoha Park-
dc.contributor.authorSujin Kim-
dc.contributor.authorMinA Kim-
dc.contributor.authorMyeong-Gyun Kang-
dc.contributor.authorChulhwan Kwak-
dc.contributor.authorMinjeong Kang-
dc.contributor.authorBaekgyu Kim-
dc.contributor.authorHyun-Woo Rhee-
dc.contributor.authorV. Narry Kim-
dc.date.available2019-01-30T02:01:23Z-
dc.date.created2018-12-26-
dc.date.issued2018-09-
dc.identifier.issn1097-2765-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/5433-
dc.description.abstractProtein kinase RNA-activated (PKR) induces immune response by sensing viral double-stranded RNAs (dsRNAs). However, growing evidence suggests that PKR can also be activated by endogenously expressed dsRNAs. Here, we capture these dsRNAs by formaldehyde-mediated crosslinking and immunoprecipitation sequencing and find that various noncoding RNAs interact with PKR. Surprisingly, the majority of the PKR-interacting RNA repertoire is occupied by mitochondrial RNAs (mtRNAs). MtRNAs can form intermolecular dsRNAs owing to bidirectional transcription of the mitochondrial genome and regulate PKR and eIF2�� phosphorylation to control cell signaling and translation. Moreover, PKR activation by mtRNAs is counteracted by PKR phosphatases, disruption of which causes apoptosis from PKR overactivation even in uninfected cells. Our work unveils dynamic regulation of PKR even without infection and establishes PKR as a sensor for nuclear and mitochondrial signaling cues in regulating cellular metabolism. By employing formaldehyde crosslinking, Kim et al. provided genome-wide analysis of cellular dsRNAs that can interact with immune response protein PKR. They identify numerous noncoding RNAs that bind to PKR and reveal mitochondrial RNAs, which exist as intermolecular dsRNAs, that can regulate PKR phosphorylation and downstream signaling, especially under stress. ? 2018 Elsevier Inc-
dc.description.uri1-
dc.language영어-
dc.publisherCELL PRESS-
dc.subjectAlu-
dc.subjectcell cycle-
dc.subjectcellular dsRNAs-
dc.subjectfCLIP-seq-
dc.subjecthigh-throughput sequencing-
dc.subjectmitochondria-
dc.subjectmitochondrial RNAs-
dc.subjectPKR-
dc.subjectretrotransposons-
dc.subjectstress response-
dc.titlePKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000445103900016-
dc.identifier.scopusid2-s2.0-85056656112-
dc.identifier.rimsid66480-
dc.contributor.affiliatedAuthorYoosik Kim-
dc.contributor.affiliatedAuthorJoha Park-
dc.contributor.affiliatedAuthorBaekgyu Kim-
dc.contributor.affiliatedAuthorV. Narry Kim-
dc.identifier.doi10.1016/j.molcel.2018.07.029-
dc.identifier.bibliographicCitationMOLECULAR CELL, v.71, no.6, pp.1051 - 1.06E+09-
dc.citation.titleMOLECULAR CELL-
dc.citation.volume71-
dc.citation.number6-
dc.citation.startPage1051-
dc.citation.endPage1.06E+09-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordAuthorstress response-
dc.subject.keywordAuthorAlu-
dc.subject.keywordAuthorcell cycle-
dc.subject.keywordAuthorcellular dsRNAs-
dc.subject.keywordAuthorfCLIP-seq-
dc.subject.keywordAuthorhigh-throughput sequencing-
dc.subject.keywordAuthormitochondria-
dc.subject.keywordAuthormitochondrial RNAs-
dc.subject.keywordAuthorPKR-
dc.subject.keywordAuthorretrotransposons-
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
1-s2.0-S1097276518305987-main.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse