BROWSE

Related Scientist

GEL's photo.

GEL
유전체 교정 연구단
more info

ITEM VIEW & DOWNLOAD

DCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy

Cited 2 time in webofscience Cited 15 time in scopus
807 Viewed 190 Downloaded
Title
DCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy
Author(s)
Hyomin Lee; Jihye Choi; Euihwan Jeong; Seongho Baek; Hee Chan Kim; Jong-Hee Chae; Youngil Koh; Sang Woo Seo; Jin-Soo Kim; Sung Jae Kim
Subject
dCas9, ; direct detection, ; Ion concentration polarization, ; liquid biopsy, ; micro/nanofluidics, ; selective preconcentration
Publication Date
2018-11
Journal
NANO LETTERS, v.18, no.12, pp.7642 - 7650
Publisher
AMER CHEMICAL SOC
Abstract
The-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapid and direct detection. Theoretical analysis on ICP phenomenon figured out a critical mobility, elucidating two distinguishable concentrating behaviors near a nanojunction, a stacking and a propagating behavior. Through the modulation of the critical mobility to shift those behaviors, the C-C chemokine receptor type 5 (CCR5) sequences were optically detected without PCR amplification. Conclusively, the proposed dCas9-mediated genetic detection methodology based on ICP would provide rapid and accurate micro/nanofluidic platform of liquid biopsies for disease diagnostics. © 2018 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/5401
DOI
10.1021/acs.nanolett.8b03224
ISSN
1530-6984
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
acs.nanolett.8b03224.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse