DCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy

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Title
DCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy
Author(s)
Hyomin Lee; Jihye Choi; Euihwan Jeong; Seongho Baek; Hee Chan Kim; Jong-Hee Chae; Youngil Koh; Sang Woo Seo; Jin-Soo Kim; Sung Jae Kim
Publication Date
2018-11
Journal
NANO LETTERS, v.18, no.12, pp.7642 - 7650
Publisher
AMER CHEMICAL SOC
Abstract
The-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapid and direct detection. Theoretical analysis on ICP phenomenon figured out a critical mobility, elucidating two distinguishable concentrating behaviors near a nanojunction, a stacking and a propagating behavior. Through the modulation of the critical mobility to shift those behaviors, the C-C chemokine receptor type 5 (CCR5) sequences were optically detected without PCR amplification. Conclusively, the proposed dCas9-mediated genetic detection methodology based on ICP would provide rapid and accurate micro/nanofluidic platform of liquid biopsies for disease diagnostics. © 2018 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/5401
ISSN
1530-6984
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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