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유전체교정연구단
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DCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy

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dc.contributor.authorHyomin Lee-
dc.contributor.authorJihye Choi-
dc.contributor.authorEuihwan Jeong-
dc.contributor.authorSeongho Baek-
dc.contributor.authorHee Chan Kim-
dc.contributor.authorJong-Hee Chae-
dc.contributor.authorYoungil Koh-
dc.contributor.authorSang Woo Seo-
dc.contributor.authorJin-Soo Kim-
dc.contributor.authorSung Jae Kim-
dc.date.available2019-01-30T01:59:38Z-
dc.date.created2018-12-26-
dc.date.issued2018-11-
dc.identifier.issn1530-6984-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/5401-
dc.description.abstractThe-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapid and direct detection. Theoretical analysis on ICP phenomenon figured out a critical mobility, elucidating two distinguishable concentrating behaviors near a nanojunction, a stacking and a propagating behavior. Through the modulation of the critical mobility to shift those behaviors, the C-C chemokine receptor type 5 (CCR5) sequences were optically detected without PCR amplification. Conclusively, the proposed dCas9-mediated genetic detection methodology based on ICP would provide rapid and accurate micro/nanofluidic platform of liquid biopsies for disease diagnostics. © 2018 American Chemical Society-
dc.description.uri1-
dc.language영어-
dc.publisherAMER CHEMICAL SOC-
dc.subjectdCas9-
dc.subjectdirect detection-
dc.subjectIon concentration polarization-
dc.subjectliquid biopsy-
dc.subjectmicro/nanofluidics-
dc.subjectselective preconcentration-
dc.titleDCas9-mediated Nanoelectrokinetic Direct Detection of Target Gene for Liquid Biopsy-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000453488800029-
dc.identifier.scopusid2-s2.0-85058082931-
dc.identifier.rimsid66438-
dc.contributor.affiliatedAuthorEuihwan Jeong-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1021/acs.nanolett.8b03224-
dc.identifier.bibliographicCitationNANO LETTERS, v.18, no.12, pp.7642 - 7650-
dc.citation.titleNANO LETTERS-
dc.citation.volume18-
dc.citation.number12-
dc.citation.startPage7642-
dc.citation.endPage7650-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordAuthordCas9-
dc.subject.keywordAuthordirect detection-
dc.subject.keywordAuthorIon concentration polarization-
dc.subject.keywordAuthorliquid biopsy-
dc.subject.keywordAuthormicro/nanofluidics-
dc.subject.keywordAuthorselective preconcentration-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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