Do osmolytes impact the structure and dynamics of myoglobin?

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Title
Do osmolytes impact the structure and dynamics of myoglobin?
Author(s)
Dorota Kossowska; Kyungwon Kwak; Minhaeng Cho
Publication Date
2018-12
Journal
MOLECULES, v.23, no.12, pp.3189 -
Publisher
MDPI AG
Abstract
Osmolytes are small organic compounds that can affect the stability of proteins in living cells. The mechanism of osmolytes�� protective effects on protein structure and dynamics has not been fully explained, but in general, two possibilities have been suggested and examined: a direct interaction of osmolytes with proteins (water replacement hypothesis), and an indirect interaction (vitrification hypothesis). Here, to investigate these two possible mechanisms, we studied myoglobin-osmolyte systems using FTIR, UV-vis, CD, and femtosecond IR pump-probe spectroscopy. Interestingly, noticeable changes are observed in both the lifetime of the CO stretch of CO-bound myoglobin and the spectra of UV-vis, CD, and FTIR upon addition of the osmolytes. In addition, the temperature-dependent CD studies reveal that the protein��s thermal stability depends on molecular structure, hydrogen-bonding ability, and size of osmolytes. We anticipate that the present experimental results provide important clues about the complicated and intricate mechanism of osmolyte effects on protein structure and dynamics in a crowded cellular environment. (c) 2018 by the authors
URI
https://pr.ibs.re.kr/handle/8788114/5386
ISSN
1420-3049
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
Files in This Item:
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