Spectral reflectometric microscopy on myelinated axons in situ

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Title
Spectral reflectometric microscopy on myelinated axons in situ
Author(s)
Junhwan Kwon; Myunghwan Choi
Publication Date
2018-07
Journal
Jove-Journal of Visualized Experiments, v.2018, no.137, pp.e57965 -
Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
Abstract
In a mammalian nervous system, myelin provides an electrical insulation by enwrapping the axon fibers in a multilayered spiral. Inspired by its highly-organized subcellular architecture, we recently developed a new imaging modality, named spectral reflectometry (SpeRe), which enables unprecedented label-free nanoscale imaging of the live myelinated axons in situ. The underlying principle is to obtain nanostructural information by analyzing the reflectance spectrum of the multilayered subcellular structure. In this article, we describe a detailed step-by-step protocol for performing a basic SpeRe imaging of the nervous tissues using a commercial confocal microscopic system, equipped with a white-light laser and a tunable filter. We cover the procedures of sample preparation, acquisition of spectral data, and image processing for obtaining nanostructural information.© 2018 Journal of Visualized Experiments
URI
https://pr.ibs.re.kr/handle/8788114/5255
ISSN
1940-087X
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
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