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나노의학 연구단
나노의학 연구단
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Three-dimensional brain-like microenvironments facilitate the direct reprogramming of fibroblasts into therapeutic neurons

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Title
Three-dimensional brain-like microenvironments facilitate the direct reprogramming of fibroblasts into therapeutic neurons
Author(s)
Yoonhee Jin; Jung Seung Lee; Jin Kim; Sungjin Min; Soohyun Wi; Ji Hea Yu; Gyeong-Eon Chang; Ann-Na Cho; Yeeun Choi; Da-Hee Ahn; Sung-Rae Cho; Eunji Cheong; Yun-Gon Kim; Hyong-Pyo Kim; Yonghwan Kim; Dong Seok Kim; Hyun Woo Kim; Zhejiu Quan; Hoon-Chul Kang; Seung-Woo Cho
Publication Date
2018-07
Journal
NATURE BIOMEDICAL ENGINEERING, v.2, no.7, pp.522 - 539
Publisher
NATURE PUBLISHING GROUP
Abstract
Biophysical cues can improve the direct reprogramming of fibroblasts into neurons that can be used for therapeutic purposes. However, the effects of a three-dimensional (3D) environment on direct neuronal reprogramming remain unexplored. Here, we show that brain extracellular matrix (BEM) decellularized from human brain tissue facilitates the plasmid-transfection-based direct conversion of primary mouse embryonic fibroblasts into induced neuronal (iN) cells. We first show that two-dimensional (2D) surfaces modified with BEM significantly increase the generation efficiency of iN cells and enhance neuronal transdifferentiation and maturation. Moreover, in an animal model of ischaemic stroke, iN cells generated on the BEM substrates and transplanted into the brain led to significant improvements in locomotive behaviours. We also show that compared with the 2D BEM substrates, 3D BEM hydrogels recapitulating brain-like microenvironments further promote neuronal conversion and potentiate the functional recovery of the animals. Our findings suggest that 3D microenvironments can boost nonviral direct reprogramming for the generation of therapeutic neuronal cells. © 2018 The Author(s)
URI
https://pr.ibs.re.kr/handle/8788114/5249
ISSN
2157-846X
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > Journal Papers
Files in This Item:
201807_Nat. Biomed. Eng._Three-dimensional brain-like microenvironments.pdfDownload

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