Longer-Lasting Electron-Based Microscopy of Single Molecules in Aqueous Medium

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Title
Longer-Lasting Electron-Based Microscopy of Single Molecules in Aqueous Medium
Author(s)
Huan Wang; K. Hima Nagamanasa; Ye-Jin Kim; Oh-Hoon Kwon; Steve Granick
Publication Date
2018-08
Journal
ACS NANO, v.12, no.8, pp.8572 - 8578
Publisher
AMER CHEMICAL SOC
Abstract
Use of electron-based microscopy in aqueous media has been held back because aqueous samples tend to suffer from water radiolysis and other chemical degradation caused by the high energy of incident electrons. Here we show that aqueous liquid pockets in graphene liquid cells at room temperature display significantly improved stability when using deuterated water, D2O. Reporting transmission electron microscopy (TEM) experiments based on common imaging conditions, we conclude that use of D2O outperforms adding radical scavengers to H2O regardless of imaging details; it increases the lifetime of dissolved organic macromolecules by a factor of 2-5, and it delays by even longer the appearance of radiolysis-induced bubbles, by a factor of time up to 10. We quantify statistically the consequences of minimizing the electron voltage and dose and conclude that the D2O environment increases sample longevity without noticeable sacrifice of contrast that is critical for direct imaging of weakly scattering organic macromolecules and biomolecules © 2018 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/5232
ISSN
1936-0851
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
Huan Wang et al_ACS Nano.pdfDownload

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