Deep line-temporal focusing with high axial resolution and a large field-of-view using intracavity control and incoherent pulse shaping

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Title
Deep line-temporal focusing with high axial resolution and a large field-of-view using intracavity control and incoherent pulse shaping
Author(s)
Kai Lou; Bo Wang; Ah-Young Jee; Steve Granick; François Amblard
Publication Date
2018-10
Journal
OPTICS LETTERS, v.43, no.20, pp.4919 - 4922
Publisher
OPTICAL SOC AMER
Abstract
Line-temporal focusing has been recognized as an elegant strategy that provides two-photon microscopy with an effective means for fast imaging through parallelization, together with an improved resilience to scattering for deep imaging. However, the axial resolution remains sub-optimal, except when using high NA objectives and a small field-of-view. With the introduction of an intracavity control of the spectral width of the femtosecond laser to adaptively fill the back aperture of the objective lens, line-temporal focusing two-photon microscopy is demonstrated to reach near-diffraction-limited axial resolution with a large back-aperture objective lens, and improved immunity to sample scattering. In addition, a new incoherent flattop beam shaping method is proposed which provides a uniform contrast with little degradation of the axial resolution along the focus line, even deep in the sample. This is demonstrated in large volumetric imaging of mouse lung samples. © 2018 Optical Society of America
URI
https://pr.ibs.re.kr/handle/8788114/5146
ISSN
0146-9592
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
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