Fully automated, on-site isolation of cfDNA from whole blood for cancer therapy monitoring

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Title
Fully automated, on-site isolation of cfDNA from whole blood for cancer therapy monitoring
Author(s)
Chi-Ju Kim; Juhee Park; Vijaya Sunkara; Tae-Hyeong Kim; Yongjin Lee; Kyusang Lee; Mi-Hyun Kim; Yoon-Kyoung Cho
Publication Date
2018-05
Journal
LAB ON A CHIP, v.18, no.9, pp.1320 - 1329
Publisher
ROYAL SOC CHEMISTRY
Abstract
The potential utility of circulating tumour DNA (ctDNA) in patient blood for cancer diagnostics and real-time monitoring of disease progression is highly recognized. However, the lack of automated and efficient methods for cell-free DNA (cfDNA) isolation from peripheral blood has remained a challenge for broader acceptance of liquid biopsy in general clinical settings. Here, we demonstrate a lab-on-a-disc system equipped with newly developed, electromagnetically actuated, and reversible diaphragm valves that allows fully automated and rapid (<30 min) isolation of cfDNA from whole blood (>3 ml) to achieve high detection sensitivity by minimizing the degradation of fragile ctDNA as well as contamination of wild-type DNA from abundant blood cells. As a proof of concept study, we used the lab-on-a-disc to isolate cfDNA from patients with non-small cell lung cancer and successfully detected epidermal growth factor receptor gene mutations (L858R, T790M) during targeted drug therapy. The proposed lab-on-a-disc enables a fully automated, rapid, and point-of-care cfDNA enrichment starting from whole blood to facilitate the wide use of liquid biopsy in routine clinical practice © The Royal Society of Chemistry 2018
URI
https://pr.ibs.re.kr/handle/8788114/4986
ISSN
1473-0197
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
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