Label-free and live cell imaging by interferometric scattering microscopy

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Title
Label-free and live cell imaging by interferometric scattering microscopy
Author(s)
Jin-Sung Park; Il-Buem Lee; Hyeon-Min Moon; Jong-Hyeon Joo; Kyoung-Hoon Kim; Seok-Cheol Hong; Minhaeng Cho
Publication Date
2018-03
Journal
Chemical Science, v.9, no.10, pp.2690 - 2697
Publisher
ROYAL SOC CHEMISTRY
Abstract
Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, Escherischia coli, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact areas of the cells attached onto a glass substrate, e.g., focal adhesions and filopodia, are clearly discernible. We also found a variety of fringe-like features in the cytoplasmic area, which may reflect the folded structures of cytoplasmic organelles. We thus anticipate that the label-free interferometric scattering microscopy can be used as a powerful tool to shed interferometric light on in vivo structures and dynamics of various intracellular phenomena. © 2018 The Royal Society of Chemistry
URI
https://pr.ibs.re.kr/handle/8788114/4608
ISSN
2041-6520
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
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