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강범창
유전체 교정 연구단
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Precision genome engineering through adenine base editing in plants

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Title
Precision genome engineering through adenine base editing in plants
Author(s)
Beum-Chang Kang; Jae-Young Yun; Sang-Tae Kim; YouJin Shin; Jahee Ryu; Minkyung Choi; Je Wook Woo; Jin-Soo Kim
Publication Date
2018-07
Journal
Nature Plants, v.4, no.7, pp.427 - 431
Publisher
NATURE PUBLISHING GROUP
Abstract
The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide ‘proof of concept’ for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology. © 2018 The Author(s
URI
https://pr.ibs.re.kr/handle/8788114/4491
ISSN
2055-026X
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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