BROWSE

Related Scientist

kang,beumchang's photo.

kang,beumchang
유전체교정연구단
more info

ITEM VIEW & DOWNLOAD

Precision genome engineering through adenine base editing in plantsHighly Cited Paper

DC Field Value Language
dc.contributor.authorBeum-Chang Kang-
dc.contributor.authorJae-Young Yun-
dc.contributor.authorSang-Tae Kim-
dc.contributor.authorYouJin Shin-
dc.contributor.authorJahee Ryu-
dc.contributor.authorMinkyung Choi-
dc.contributor.authorJe Wook Woo-
dc.contributor.authorJin-Soo Kim-
dc.date.available2018-07-18T02:02:19Z-
dc.date.created2018-06-26-
dc.date.issued2018-07-
dc.identifier.issn2055-026X-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/4491-
dc.description.abstractThe recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide ‘proof of concept’ for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology. © 2018 The Author(s-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.titlePrecision genome engineering through adenine base editing in plants-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000443221200014-
dc.identifier.scopusid2-s2.0-85048029625-
dc.identifier.rimsid63856ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorBeum-Chang Kang-
dc.contributor.affiliatedAuthorJae-Young Yun-
dc.contributor.affiliatedAuthorSang-Tae Kim-
dc.contributor.affiliatedAuthorYouJin Shin-
dc.contributor.affiliatedAuthorJahee Ryu-
dc.contributor.affiliatedAuthorMinkyung Choi-
dc.contributor.affiliatedAuthorJe Wook Woo-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1038/s41477-018-0178-x-
dc.identifier.bibliographicCitationNATURE PLANTS, v.4, no.7, pp.427 - 431-
dc.citation.titleNATURE PLANTS-
dc.citation.volume4-
dc.citation.number7-
dc.citation.startPage427-
dc.citation.endPage431-
dc.date.scptcdate2018-10-01-
dc.description.wostc1-
dc.description.scptc1-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusDEAMINASE FUSION-
dc.subject.keywordPlusDNA CLEAVAGE-
dc.subject.keywordPlusARABIDOPSIS-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusWHEAT-
dc.subject.keywordPlusRICE-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
2018_강범창_NPlants.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse