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분자 분광학 및 동력학 연구단
분자 분광학 및 동력학 연구단
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Optical imaging featuring both long working distance and high spatial resolution by correcting the aberration of a large aperture lens

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Title
Optical imaging featuring both long working distance and high spatial resolution by correcting the aberration of a large aperture lens
Author(s)
Changsoon Choi; Kyung-Deok Song; Sungsam Kang; Jin-Sung Park; Wonshik Choi
Publication Date
2018-06
Journal
SCIENTIFIC REPORTS, v.8, no.1, pp.9165 -
Publisher
NATURE PUBLISHING GROUP
Abstract
High-resolution optical imaging within thick objects has been a challenging task due to the short working distance of conventional high numerical aperture (NA) objective lenses. Lenses with a large physical diameter and thus a large aperture, such as microscope condenser lenses, can feature both a large NA and a long working distance. However, such lenses suffer from strong aberrations. To overcome this problem, we present a method to correct the aberrations of a transmission-mode imaging system that is composed of two condensers. The proposed method separately identifies and corrects aberrations of illumination and collection lenses of up to 1.2 NA by iteratively optimizing the total intensity of the synthetic aperture images in the forward and phase-conjugation processes. At a source wavelength of 785 nm, we demonstrated a spatial resolution of 372 nm at extremely long working distances of up to 1.6 mm, an order of magnitude improvement in comparison to conventional objective lenses. Our method of converting microscope condensers to high-quality objectives may facilitate increases in the imaging depths of super-resolution and expansion microscopes. © The Author(s) 2018
URI
https://pr.ibs.re.kr/handle/8788114/4485
ISSN
2045-2322
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
Files in This Item:
[2018] Scientific_Reports Transmission CLASS.pdfDownload

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