BROWSE

Related Scientist

Researcher

분자 분광학 및 동력학 연구단
분자 분광학 및 동력학 연구단
more info

Effect of Osmolytes on the Conformational Behavior of a Macromolecule in a Cytoplasm-like Crowded Environment: A Femtosecond Mid-IR Pump–Probe Spectroscopy Study

Cited 4 time in webofscience Cited 0 time in scopus
137 Viewed 6 Downloaded
Title
Effect of Osmolytes on the Conformational Behavior of a Macromolecule in a Cytoplasm-like Crowded Environment: A Femtosecond Mid-IR Pump–Probe Spectroscopy Study
Author(s)
Achintya Kundu; Pramod Kumar Verma; Minhaeng Cho
Publication Date
2018-02
Journal
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, v.9, no.4, pp.724 - 731
Publisher
AMER CHEMICAL SOC
Abstract
Osmolytes found endogenously in almost all living beings play an important role in regulating cell volume under harsh environment. Here, to address the longstanding questions about the underlying mechanism of osmolyte effects, we use femtosecond mid-IR pump–probe spectroscopy with two different IR probes that are the OD stretching mode of HDO and the azido stretching mode of azido-derivatized poly(ethylene glycol) dimethyl ether (PEGDME). Our experimental results show that protecting osmolytes bind strongly with water molecules and dehydrate polymer surface, which results in promoting intramolecular interactions of the polymer. By contrast, urea behaves like water molecules without significantly disrupting water H-bonding network and favors extended and random-coil segments of the polymer chain by directly participating in solvation of the polymer. Our findings highlight the importance of direct interaction between urea and macromolecule, while protecting osmolytes indirectly affect the macromolecule through enhancing the water–osmolyte interaction in a crowded environment, which is the case that is often encountered in real biological systems. © XXXX American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/4444
ISSN
1948-7185
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
Files in This Item:
Effect of Osmolytes on the Conformational Behavior of a Macromolecule_Achintya(조민행).pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse