Spatiotemporal Control of TGF-β Signaling with Light

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Title
Spatiotemporal Control of TGF-β Signaling with Light
Author(s)
Yuchao Li; Minji Lee; Nury Kim; Guoyu Wu; Difan Deng; Jin Man Kim; Xuedong Liu; Won Do Heo; Zhike Zi
Publication Date
2018-02
Journal
ACS Synthetic Biology, v.7, no.2, pp.443 - 451
Publisher
AMER CHEMICAL SOC
Abstract
Cells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-β) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-β signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-β signaling with high spatiotemporal resolution. Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space. Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations. By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations. The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level. © 2018 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/4376
ISSN
2161-5063
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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