Nanomagnetic System for Rapid Diagnosis of Acute Infection

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Title
Nanomagnetic System for Rapid Diagnosis of Acute Infection
Author(s)
Ki Soo Park; Hoyoung Kim; Soojin Kim; Kyungheon Lee; Sohyeon Park; Jun Song; Changwook Min; Farhana Khanam; Rasheduzzaman Rashu; Taufiqur Rahman Bhuiyan; Edward T.Ryan; Firdausi Qadri; Ralph Weissleder; Jinwoo Cheon; Richelle C. Charles; Hakho Lee
Publication Date
2017-11
Journal
ACS NANO, v.11, no.11, pp.11425 - 11432
Publisher
AMER CHEMICAL SOC
Abstract
Pathogen-activated antibody-secreting cells (ASCs) produce and secrete antigen-specific antibodies. ASCs are detectable in the peripheral blood as early as 3 days after antigen exposure, which makes ASCs a potential biomarker for early disease detection. Here, we present a magnetic capture and detection (MCD) assay for sensitive, on-site detection of ASCs. In this approach, ASCs are enriched through magnetic capture, and secreted antibodies are magnetically detected by a miniaturized nuclear magnetic resonance (μNMR) system. This approach is based entirely on magnetics, which supports high contrast against biological background and simplifies assay procedures. We advanced the MCD system by (i) synthesizing magnetic nanoparticles with high magnetic moments for both cell capture and antibody detection, (ii) developing a miniaturized magnetic device for high-yield cell capture, and (iii) optimizing the μNMR assay for antibody detection. Antibody responses targeting hemolysin E (HlyE) can accurately identify individuals with acute enteric fever. As a proof-of-concept, we applied MCD to detect antibodies produced by HlyE-specific hybridoma cells. The MCD achieved high sensitivity in detecting antibodies secreted from as few as 5 hybridoma cells (50 cells/mL). Importantly, the assay could be performed with whole blood with minimal sample processing. © 2017 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/4256
ISSN
1936-0851
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > Journal Papers
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