Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

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Title
Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes
Author(s)
Sanjaya C. Abeysirigunawardena; Hajin Kim; Jonathan Lai; Kaushik Ragunathan; Mollie C. Rappé; Zaida Luthey-Schulten; Taekjip Ha; Sarah A. Woodson
Publication Date
2017-09
Journal
NATURE COMMUNICATIONS, v.8, no.1, pp.492 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Assembly of 30S ribosomes involves the hierarchical addition of ribosomal proteins that progressively stabilize the folded 16S rRNA. Here, we use three-color single molecule FRET to show how combinations of ribosomal proteins uS4, uS17 and bS20 in the 16S 5' domain enable the recruitment of protein bS16, the next protein to join the complex. Analysis of real-time bS16 binding events shows that bS16 binds both native and non-native forms of the rRNA. The native rRNA conformation is increasingly favored after bS16 binds, explaining how bS16 drives later steps of 30S assembly. Chemical footprinting and molecular dynamics simulations show that each ribosomal protein switches the 16S conformation and dampens fluctuations at the interface between rRNA subdomains where bS16 binds. The results suggest that specific protein-induced changes in the rRNA dynamics underlie the hierarchy of 30S assembly and simplify the search for the native ribosome structure. © The Author(s) 2017
URI
https://pr.ibs.re.kr/handle/8788114/4172
ISSN
2041-1723
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > Journal Papers (저널논문)
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