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Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2

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Title
Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2
Author(s)
Hyerim Park; Na Yeon Kim; Sangkyu Lee; Nury Kim; Jihoon Kim; Won Do Heo
Publication Date
2017-06
Journal
NATURE COMMUNICATIONS, v.8, no.1, pp.30 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Protein homo-oligomerization is an important molecular mechanism in many biological processes. Therefore, the ability to control protein homo-oligomerization allows the manipulation and interrogation of numerous cellular events. To achieve this, cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization. However, limited knowledge on molecular characteristics of CRY2 obscures its widespread applications. Here, we identify important determinants for efficient cryptochrome 2 clustering and introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins by employing diverse fluorescent proteins and an extremely short peptide. Furthermore, we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools. Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules. © 2017 The Author(s)
URI
http://pr.ibs.re.kr/handle/8788114/4014
ISSN
2041-1723
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
201706 Nature Communications.pdfDownload

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