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A microfluidic chip for screening individual cancer cells via eavesdropping on autophagyinducing crosstalk in the stroma niche

Cited 11 time in webofscience Cited 13 time in scopus
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Title
A microfluidic chip for screening individual cancer cells via eavesdropping on autophagyinducing crosstalk in the stroma niche
Author(s)
Hacer Ezgi Karakas; Junyoung Kim; Juhee Park; Jung Min Oh; Yongjun Choi; Devrim Gozuacik; Yoon-Kyoung Cho
Subject
Biomaterials–cells,Cancer microenvironment,Cancer models
Publication Date
2017-05
Journal
SCIENTIFIC REPORTS, v.7, pp.2050
Publisher
NATURE PUBLISHING GROUP
Abstract
Autophagy is a cellular homeostatic mechanism where proteins and organelles are digested and recycled to provide an alternative source of building blocks and energy to cells. The role of autophagy in cancer microenvironment is still poorly understood. Here, we present a microfluidic system allowing monitoring of the crosstalk between single cells. We used this system to study how tumor cells induced autophagy in the stromal niche. Firstly, we could confirm that transforming growth factor beta 1 (TGF beta 1) secreted from breast tumor cells is a paracrine mediator of tumor-stroma interaction leading to the activation of autophagy in the stroma component fibroblasts. Through proof of concept experiments using TGF beta 1 as a model factor, we could demonstrate real time monitoring of autophagy induction in fibroblasts by single tumor cells. Retrieval of individual tumor cells from the microfluidic system and their subsequent genomic analysis was possible, allowing us to determine the nature of the factor mediating tumor-stroma interactions. Therefore, our microfluidic platform might be used as a promising tool for quantitative investigation of tumor-stroma interactions, especially for and high-throughput screening of paracrine factors that are secreted from heterogeneous tumor cell populations. © The Author(s) 2017
URI
https://pr.ibs.re.kr/handle/8788114/3694
DOI
10.1038/s41598-017-02172-7
ISSN
2045-2322
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
2017_Sci. Rep._A microfluidic chip for screening individual cancer cells.pdfDownload

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