BROWSE

Related Scientist

Researcher

박주희
첨단연성물질 연구단
more info

A microfluidic chip for screening individual cancer cells via eavesdropping on autophagyinducing crosstalk in the stroma niche

Cited 0 time in webofscience Cited 0 time in scopus
167 Viewed 47 Downloaded
Title
A microfluidic chip for screening individual cancer cells via eavesdropping on autophagyinducing crosstalk in the stroma niche
Author(s)
Hacer Ezgi Karakas; Junyoung Kim; Juhee Park; Jung Min Oh; Yongjun Choi; Devrim Gozuacik; Yoon-Kyoung Cho
Publication Date
2017-05
Journal
SCIENTIFIC REPORTS, v.7, no., pp.2050 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Autophagy is a cellular homeostatic mechanism where proteins and organelles are digested and recycled to provide an alternative source of building blocks and energy to cells. The role of autophagy in cancer microenvironment is still poorly understood. Here, we present a microfluidic system allowing monitoring of the crosstalk between single cells. We used this system to study how tumor cells induced autophagy in the stromal niche. Firstly, we could confirm that transforming growth factor beta 1 (TGF beta 1) secreted from breast tumor cells is a paracrine mediator of tumor-stroma interaction leading to the activation of autophagy in the stroma component fibroblasts. Through proof of concept experiments using TGF beta 1 as a model factor, we could demonstrate real time monitoring of autophagy induction in fibroblasts by single tumor cells. Retrieval of individual tumor cells from the microfluidic system and their subsequent genomic analysis was possible, allowing us to determine the nature of the factor mediating tumor-stroma interactions. Therefore, our microfluidic platform might be used as a promising tool for quantitative investigation of tumor-stroma interactions, especially for and high-throughput screening of paracrine factors that are secreted from heterogeneous tumor cell populations. © The Author(s) 2017
URI
http://pr.ibs.re.kr/handle/8788114/3694
DOI
10.1038/s41598-017-02172-7
ISSN
2045-2322
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
2017_Sci. Rep._A microfluidic chip for screening individual cancer cells.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse