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Kinetics of the E46Q mutant of photoactive yellow protein investigated by transient grating spectroscopy

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Title
Kinetics of the E46Q mutant of photoactive yellow protein investigated by transient grating spectroscopy
Author(s)
Cheolhee Yang; Tae Wu Kim; Youngmin Kim; Jungkweon Choi; Sang Jin Lee; Hyotcherl Ihee
Publication Date
2017-09
Journal
CHEMICAL PHYSICS LETTERS, v.683, no., pp.262 - 267
Publisher
ELSEVIER SCIENCE BV
Abstract
To elucidate the role of internal proton transfer in the photodynamics of photoactive yellow protein (PYP), the photocycle of the Glu46Gln mutant of PYP (E46Q-PYP) is investigated by transient grating (TG) spectroscopy. Compared with wild-type PYP (wt-PYP), the first spectrally blue-shifted intermediate of E46Q-PYP is formed more slowly, which is consistent with the absence of direct protonation from Glu46 residue, if the parallel kinetic model for wt-PYP is invoked. The smaller conformational change in E46Q-PYP, as manifested by the smaller change in the diffusion coefficient, mainly arises from the relatively larger volume of the ground state E46Q-PYP than wt-PYP rather than from the smaller volume of the pB state. (c) 2017 Elsevier B.V. All rights reserved.
URI
https://pr.ibs.re.kr/handle/8788114/3641
ISSN
0009-2614
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > Journal Papers (저널논문)
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