In vivo patch-clamp recording in awake head-fixed rodents

Cited 0 time in webofscience Cited 0 time in scopus
146 Viewed 8 Downloaded
Title
In vivo patch-clamp recording in awake head-fixed rodents
Author(s)
Doyun Lee; Albert K. Lee
Publication Date
2017-04
Journal
Cold Spring Harbor Protocols, v.2017, no.4, pp.274 - 280
Publisher
Cold Spring Harbor Laboratory Press
Abstract
Whole-cell recording has been used to measure and manipulate a neuron’s spiking and subthreshold membrane potential, allowing assessment of the cell’s inputs and outputs as well as its intrinsic membrane properties. This technique has also been combined with pharmacology and optogenetics as well as morphological reconstruction to address critical questions concerning neuronal integration, plasticity, and connectivity. This protocol describes a technique for obtaining whole-cell recordings in awake head-fixed animals, allowing such questions to be investigated within the context of an intact network and natural behavioral states. First, animals are habituated to sit quietly with their heads fixed in place. Then, a whole-cell recording is obtained using an efficient, blind patching protocol. We have successfully applied this technique to rats and mice. © 2017 Cold Spring Harbor Laboratory Press
URI
https://pr.ibs.re.kr/handle/8788114/3583
ISSN
1940-3402
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
Lee(CSHP17b).pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse