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In vivo patch-clamp recording in awake head-fixed rodents

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dc.contributor.authorDoyun Lee-
dc.contributor.authorAlbert K. Lee-
dc.date.available2017-05-30T06:08:50Z-
dc.date.created2017-05-19-
dc.date.issued2017-04-
dc.identifier.issn1940-3402-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/3583-
dc.description.abstractWhole-cell recording has been used to measure and manipulate a neuron’s spiking and subthreshold membrane potential, allowing assessment of the cell’s inputs and outputs as well as its intrinsic membrane properties. This technique has also been combined with pharmacology and optogenetics as well as morphological reconstruction to address critical questions concerning neuronal integration, plasticity, and connectivity. This protocol describes a technique for obtaining whole-cell recordings in awake head-fixed animals, allowing such questions to be investigated within the context of an intact network and natural behavioral states. First, animals are habituated to sit quietly with their heads fixed in place. Then, a whole-cell recording is obtained using an efficient, blind patching protocol. We have successfully applied this technique to rats and mice. © 2017 Cold Spring Harbor Laboratory Press-
dc.language영어-
dc.publisherCold Spring Harbor Laboratory Press-
dc.titleIn vivo patch-clamp recording in awake head-fixed rodents-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.scopusid2-s2.0-85017233970-
dc.identifier.rimsid59507ko
dc.date.tcdate2017-05-19-
dc.contributor.affiliatedAuthorDoyun Lee-
dc.identifier.doi10.1101/pdb.prot095802-
dc.identifier.bibliographicCitationCold Spring Harbor Protocols, v.2017, no.4, pp.274 - 280-
dc.relation.isPartOfCold Spring Harbor Protocols-
dc.citation.titleCold Spring Harbor Protocols-
dc.citation.volume2017-
dc.citation.number4-
dc.citation.startPage274-
dc.citation.endPage280-
dc.date.scptcdate2018-10-01-
dc.description.scptc2-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
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