BROWSE

Related Scientist

nanomat's photo.

nanomat
나노입자연구단
more info

ITEM VIEW & DOWNLOAD

In Vivo Micro-CT Imaging of Human Mesenchymal Stem Cells Labeled with Gold-Poly-l-Lysine Nanocomplexes

DC Field Value Language
dc.contributor.authorTaeho Kim-
dc.contributor.authorNohyun Lee-
dc.contributor.authorArifin, DR-
dc.contributor.authorShats, I-
dc.contributor.authorJanowski, M-
dc.contributor.authorWalczak, P-
dc.contributor.authorTaeghwan Hyeon-
dc.contributor.authorBulte, JWM-
dc.date.available2017-05-19T01:13:36Z-
dc.date.created2017-02-24-
dc.date.issued2017-01-
dc.identifier.issn1616-301X-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/3497-
dc.description.abstractDeveloping in vivo cell tracking is an important prerequisite for further development of cell-based therapy. So far, few computed tomography (CT) cell tracking studies have been described due to its notoriously low sensitivity and lack of efficient labeling protocols. A simple method is presented to render human mesenchymal stem cells (hMSCs) sufficiently radiopaque by complexing 40 nm citrate-stabilized gold nanoparticles (AuNPs) with poly-l-lysine (PLL) and rhodamine B isothiocyanate (RITC). AuNP-PLL-RITC labeling does not affect cellular viability, proliferation, or downstream cell differentiation into adipocytes and osteocytes. Labeled hMSCs can be clearly visualized in vitro and in vivo with a micro-CT scanner, with a detection limit of approximate to 2 x 10(4) cells per mu L in vivo. Calculated Hounsfield unit values are 2.27 per pg of intracellular Au, as measured with inductively coupled plasma mass spectrophotometry, and are linear over a wide range of cell concentrations. This linear CT attenuation is observed for both naked AuNPs and those that were taken up by hMSCs, indicating that the number of labeled cells can be quantified similar to the use of radioactive or fluorine tracers. This approach for CT cell tracking may find applications in CT image-guided interventions and fluoroscopic procedures commonly used for the injection of cellular therapeutics. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim-
dc.description.uri1-
dc.language영어-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.titleIn Vivo Micro-CT Imaging of Human Mesenchymal Stem Cells Labeled with Gold-Poly-l-Lysine Nanocomplexes-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000391926700008-
dc.identifier.scopusid2-s2.0-85006172891-
dc.identifier.rimsid58815ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorTaeho Kim-
dc.contributor.affiliatedAuthorNohyun Lee-
dc.contributor.affiliatedAuthorTaeghwan Hyeon-
dc.identifier.doi10.1002/adfm.201604213-
dc.identifier.bibliographicCitationADVANCED FUNCTIONAL MATERIALS, v.27, no.3, pp.1604213-
dc.citation.titleADVANCED FUNCTIONAL MATERIALS-
dc.citation.volume27-
dc.citation.number3-
dc.citation.startPage1604213-
dc.date.scptcdate2018-10-01-
dc.description.wostc16-
dc.description.scptc17-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusRAY COMPUTED-TOMOGRAPHY-
dc.subject.keywordPlusCONTRAST AGENTS-
dc.subject.keywordPlusMAGNETIC-RESONANCE-
dc.subject.keywordPlusTRANSFECTION AGENTS-
dc.subject.keywordPlusOXIDE NANOPARTICLES-
dc.subject.keywordPlusPROGENITOR CELLS-
dc.subject.keywordPlusT-CELLS-
dc.subject.keywordPlusMRI-
dc.subject.keywordPlusQUANTIFICATION-
dc.subject.keywordPlusNANOMEDICINE-
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
Advanced Functional Mat(in vivo micro).pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse