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James Murray
복잡계 자기조립 연구단
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Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule

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Title
Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule
Author(s)
James Murray; Sim, J; Oh, K; Sung, G; Lee, A; Annadka Shrinidhi; Ayyavu Thirunarayanan; Dinesh Shetty; Kimoon Kim
Publication Date
2017-02
Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.56, no.9, pp.2395 - 2398
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Chemical proteomics relies primarily on click-chemistry-based protein labeling and biotin-streptavidin enrichment, but these techniques have inherent limitations. Enrichment of intracellular proteins using a totally synthetic host-guest complex is described, overcoming the problem associated with the classical approach. We achieve this by affinity-based protein labeling with a target-specific probe molecule conjugated to a high-affinity guest (suberanilohydroxamic acid-ammonium-adamantane; SAHA-Ad) and then enriching the labeled species using a cucurbit[7]uril bead. This method shows high specificity for labeled molecules in a MDA-MB-231 breast cancer cell lysate. Moreover, this method shows promise for labeling proteins in live cells ⓒ 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
URI
https://pr.ibs.re.kr/handle/8788114/3468
ISSN
1433-7851
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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