Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module Highly Cited Paper

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Title
Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module
Author(s)
Nambin Yim; Seung-Wook Ryu; Kyungsun Choi; Kwang Ryeol Lee; Seunghee Lee; Hojun Choi; Jeongjin Kim; Mohammed R. Shaker; Woong Sun; Ji-Ho Park; Daesoo Kim; Won Do Heo; Chulhee Choi
Publication Date
2016-07
Journal
NATURE COMMUNICATIONS, v.7, no., pp.12277 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues. © The Author(s) 2016
URI
https://pr.ibs.re.kr/handle/8788114/2743
ISSN
2041-1723
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
201607 Nature Communications.pdfDownload

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