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인지및사회성연구단
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Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction moduleHighly Cited Paper

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dc.contributor.authorNambin Yim-
dc.contributor.authorSeung-Wook Ryu-
dc.contributor.authorKyungsun Choi-
dc.contributor.authorKwang Ryeol Lee-
dc.contributor.authorSeunghee Lee-
dc.contributor.authorHojun Choi-
dc.contributor.authorJeongjin Kim-
dc.contributor.authorMohammed R. Shaker-
dc.contributor.authorWoong Sun-
dc.contributor.authorJi-Ho Park-
dc.contributor.authorDaesoo Kim-
dc.contributor.authorWon Do Heo-
dc.contributor.authorChulhee Choi-
dc.date.available2016-08-23T04:24:06Z-
dc.date.created2016-08-19-
dc.date.issued2016-07-
dc.identifier.issn2041-1723-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/2743-
dc.description.abstractNanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues. © The Author(s) 2016-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleExosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000380843100001-
dc.identifier.scopusid2-s2.0-84979200310-
dc.identifier.rimsid56298ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorWon Do Heo-
dc.identifier.doi10.1038/ncomms12277-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, v.7, pp.12277-
dc.citation.titleNATURE COMMUNICATIONS-
dc.citation.volume7-
dc.citation.startPage12277-
dc.date.scptcdate2018-10-01-
dc.description.wostc46-
dc.description.scptc48-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusMAMMALIAN-CELLS-
dc.subject.keywordPlusLIVE CELLS-
dc.subject.keywordPlusBAX-
dc.subject.keywordPlusTRANSDUCTION-
dc.subject.keywordPlusAPOPTOSIS-
dc.subject.keywordPlusLIGHT-
dc.subject.keywordPlusARABIDOPSIS-
dc.subject.keywordPlusINDUCTION-
dc.subject.keywordPlusCYTOSOL-
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
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201607 Nature Communications.pdfDownload

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