Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking

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Title
Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking
Author(s)
Mai Khanh Nguyen; Cha Yeon Kim; Jin Man Kim; Byung Ouk Park; Sangkyu Lee; Hyerim Park; Won Do Heo
Publication Date
2016-06
Journal
NATURE CHEMICAL BIOLOGY, v.12, no.6, pp.431 - 436
Publisher
NATURE PUBLISHING GROUP
Abstract
Intracellular membrane trafficking, which is involved in diverse cellular processes, is dynamic and difficult to study in a spatiotemporal manner. Here we report an optogenetic strategy, termed light-activated reversible inhibition by assembled trap of intracellular membranes (IM-LARIAT), that uses various Rab GTPases combined with blue-light-induced hetero-interaction between cryptochrome 2 and CIB1. In this system, illumination induces a rapid and reversible intracellular membrane aggregation that disrupts the dynamics and functions of the targeted membrane. We applied IM-LARIAT to specifically perturb several Rab-mediated trafficking processes, including receptor transport, protein sorting and secretion, and signaling initiated from endosomes. We finally used this tool to reveal different functions of local Rab5-mediated and Rab11-mediated membrane trafficking in growth cones and soma of young hippocampal neurons. Our results show that IM-LARIAT is a versatile tool that can be used to dissect spatiotemporal functions of intracellular membranes in diverse systems. © 2016 Nature America, Inc. All rights reserved
URI
https://pr.ibs.re.kr/handle/8788114/2602
ISSN
1552-4450
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
201604 Nature Chemical Biology.pdfDownload

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