BROWSE

Related Scientist

cnir's photo.

cnir
뇌과학이미징연구단
more info

ITEM VIEW & DOWNLOAD

Two-photon excited photoconversion of cyanine-based dyes

Cited 7 time in webofscience Cited 6 time in scopus
1,357 Viewed 596 Downloaded
Title
Two-photon excited photoconversion of cyanine-based dyes
Author(s)
Sheldon J. J. Kwok; Myunghwan Choi; Brijesh Bhayana; Xueli Zhang; Chongzhao Ran; Seok-Hyun Yun
Publication Date
2016-03
Journal
SCIENTIFIC REPORTS, v.6, pp.23866
Publisher
NATURE PUBLISHING GROUP
Abstract
The advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue.
URI
https://pr.ibs.re.kr/handle/8788114/2548
DOI
10.1038/srep23866
ISSN
2045-2322
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
Two-photon excited photoconversion of cyanine-based dyes.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse