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Two-photon excited photoconversion of cyanine-based dyes

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Title
Two-photon excited photoconversion of cyanine-based dyes
Author(s)
Sheldon J. J. Kwok; Myunghwan Choi; Brijesh Bhayana; Xueli Zhang; Chongzhao Ran; Seok-Hyun Yun
Publication Date
2016-03
Journal
SCIENTIFIC REPORTS, v.6, no., pp.23866 -
Publisher
NATURE PUBLISHING GROUP
Abstract
The advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue.
URI
http://pr.ibs.re.kr/handle/8788114/2548
DOI
10.1038/srep23866
ISSN
2045-2322
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
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