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Two-photon excited photoconversion of cyanine-based dyes

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dc.contributor.authorSheldon J. J. Kwok-
dc.contributor.authorMyunghwan Choi-
dc.contributor.authorBrijesh Bhayana-
dc.contributor.authorXueli Zhang-
dc.contributor.authorChongzhao Ran-
dc.contributor.authorSeok-Hyun Yun-
dc.date.accessioned2016-06-22T08:13:47Z-
dc.date.available2016-06-22T08:13:47Z-
dc.date.created2016-05-17-
dc.date.issued2016-03-
dc.identifier.issn2045-2322-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/2548-
dc.description.abstractThe advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue.-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleTwo-photon excited photoconversion of cyanine-based dyes-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000373170400001-
dc.identifier.scopusid2-s2.0-84963649705-
dc.identifier.rimsid55378-
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorMyunghwan Choi-
dc.identifier.doi10.1038/srep23866-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, v.6, pp.23866-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.citation.titleSCIENTIFIC REPORTS-
dc.citation.volume6-
dc.citation.startPage23866-
dc.date.scptcdate2018-10-01-
dc.description.wostc4-
dc.description.scptc2-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryMultidisciplinary Sciences-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusFLUORESCENT PROTEINS-
dc.subject.keywordPlusLIVING CELLS-
dc.subject.keywordPlusSUPERRESOLUTION MICROSCOPY-
dc.subject.keywordPlusCROSS-SECTIONS-
dc.subject.keywordPlusONE-PHOTON-
dc.subject.keywordPlusSINGLE-
dc.subject.keywordPlusPROBES-
dc.subject.keywordPlusFLUOROPHORES-
dc.subject.keywordPlusTRACKING-
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > 1. Journal Papers (저널논문)
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