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Fully automated centrifugal microfluidic device for ultrasensitive protein detection from whole blood

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Title
Fully automated centrifugal microfluidic device for ultrasensitive protein detection from whole blood
Author(s)
Park Y.-S.; Sunkara V.; Kim Y.; Lee W.S.; Han J.-R.; Yoon-Kyoung Cho
Publication Date
2016-04
Journal
JOURNAL OF VISUALIZED EXPERIMENTS, v.2016, no.110, pp.e54143 -
Publisher
MYJoVE Corporation
Abstract
Enzyme-linked immunosorbent assay (ELISA) is a promising method to detect small amount of proteins in biological samples. The devices providing a platform for reduced sample volume and assay time as well as full automation are required for potential use in point-of-carediagnostics. Recently, we have demonstrated ultrasensitive detection of serum proteins, C-reactive protein (CRP) and cardiac troponin I (cTnI), utilizing a lab-on-a-disc composed of TiO2 nanofibrous (NF) mats. It showed a large dynamic range with femto molar (fM) detection sensitivity, from a small volume of whole blood in 30 min. The device consists of several components for blood separation, metering, mixing, and washing that are automated for improved sensitivity from low sample volumes. Here, in the video demonstration, we show the experimental protocols and know-how for the fabrication of NFs as well as the disc, their integration and the operation in the following order: processes for preparing TiO2 NF mat; transfer-printing of TiO2 NF mat onto the disc; surface modification for immune-reactions, disc assembly and operation; on-disc detection and representative results for immunoassay. Use of this device enables multiplexed analysis with minimal consumption of samples and reagents. Given the advantages, the device should find use in a wide variety of applications, and prove beneficial in facilitating the analysis of low abundant proteins. © 2016 Journal of Visualized Experiments
URI
https://pr.ibs.re.kr/handle/8788114/2519
ISSN
1940-087X
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
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