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Wide-field optical coherence microscopy of the mouse brain slice

Cited 19 time in webofscience Cited 19 time in scopus
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Title
Wide-field optical coherence microscopy of the mouse brain slice
Author(s)
Min, E; Lee, J; Vavilin, A; Sunwoo Jung; Sungwon Shin; Kim, J; Woonggyu Jung
Publication Date
2015-10
Journal
OPTICS LETTERS, v.40, no.19, pp.4420 - 4423
Publisher
OPTICAL SOC AMER
Abstract
The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was 11.2 x 7.0 mm (horizontal x vertical), and the corresponding pixel resolution was 1.2 x 1.2 mu m. OCMimages were compared to traditional histology stained with Nissl and Luxol fast blue (LFB). In particular, the orientation of the fibers was analyzed and quantified in wide-field OCM. (C) 2015 Optical Society of Americ
URI
https://pr.ibs.re.kr/handle/8788114/2284
DOI
10.1364/OL.40.004420
ISSN
0146-9592
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
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