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인지 및 사회성 연구단
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Proteome-wide characterization of signalling interactions in the hippocampal CA4/DG subfield of patients with Alzheimer's disease

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Title
Proteome-wide characterization of signalling interactions in the hippocampal CA4/DG subfield of patients with Alzheimer's disease
Author(s)
Jae Ho Kim; Julien Franck; Taewook Kang; Helmut Heinsen; Rivka Ravid; Isidro Ferrer; MI Hee Cheon; Joo Yong Lee; Jong Shin Yoo; Harry W Steinbusch; Michel Salzet; Lsabelle Fournier; Young Mok Park
Publication Date
2015-06
Journal
SCIENTIFIC REPORTS, v.5, no., pp.11138 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Alzheimer's disease (AD) is the most common form of dementia; however, mechanisms and biomarkers remain unclear. Here, we examined hippocampal CA4 and dentate gyrus subfields, which are less studied in the context of AD pathology, in post-mortem AD and control tissue to identify possible biomarkers. We performed mass spectrometry-based proteomic analysis combined with label-free quantification for identification of differentially expressed proteins. We identified 4,328 proteins, of which 113 showed more than 2-fold higher or lower expression in AD hippocampi than in control tissues. Five proteins were identified as putative AD biomarkers (MDH2, PCLO, TRRAP, YWHAZ, and MUC19 isoform 5) and were cross-validated by immunoblotting, selected reaction monitoring, and MALDI imaging. We also used a bioinformatics approach to examine upstream signalling interactions of the 113 regulated proteins. Five upstream signalling (IGF1, BDNF, ZAP70, MYC, and cyclosporin A) factors showed novel interactions in AD hippocampi. Taken together, these results demonstrate a novel platform that may provide new strategies for the early detection of AD and thus its diagnosis
URI
http://pr.ibs.re.kr/handle/8788114/2001
ISSN
2045-2322
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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