Enrichment of single neurons and defined brain regions from human brain tissue samples for subsequent proteome analysis

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Title
Enrichment of single neurons and defined brain regions from human brain tissue samples for subsequent proteome analysis
Author(s)
Mariana Molina; Simone Steinbach; Young Mok Park; Su Yeong Yun; Ana Tereza Di Lorenzo Alho; Helmut Heinsen; Lea T. Grinberg; Katrin Marcus; renata E. Paraizo Leite; caroline May
Publication Date
2015-07
Journal
JOURNAL OF NEURAL TRANSMISSION, v.122, no.7, pp.993 - 1005
Publisher
SPRINGER WIEN
Abstract
Brain function in normal aging and neurological diseases has long been a subject of interest. With current technology, it is possible to go beyond descriptive analyses to characterize brain cell populations at the molecular level. However, the brain comprises over 100 billion highly specialized cells, and it is a challenge to discriminate different cell groups for analyses. Isolating intact neurons is not feasible with traditional methods, such as tissue homogenization techniques. The advent of laser microdissection techniques promises to overcome previous limitations in the isolation of specific cells. Here, we provide a detailed protocol for isolating and analyzing neurons from postmortem human brain tissue samples. We describe a workflow for successfully freezing, sectioning and staining tissue for laser microdissection. This protocol was validated by mass spectrometric analysis. Isolated neurons can also be employed for western blotting or PCR. This protocol will enable further examinations of brain cell-specific molecular pathways and aid in elucidating distinct brain functions. © 2015, Springer-Verlag Wien
URI
https://pr.ibs.re.kr/handle/8788114/1991
ISSN
0300-9564
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
Files in This Item:
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