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Unraveling the mechanistic features of RNA polymerase II termination by the 5 '-3 ' exoribonuclease Rat1

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Title
Unraveling the mechanistic features of RNA polymerase II termination by the 5 '-3 ' exoribonuclease Rat1
Author(s)
Jieun Park; Myungjin Kang; Minkyu Kim
Publication Date
2015-03
Journal
NUCLEIC ACIDS RESEARCH, v.43, no.5, pp.2625 - 2637
Publisher
OXFORD UNIV PRESS
Abstract
Within a complex with Rai1, the 5'-3' exoribonuclease Rat1 promotes termination of RNA polymerase II (RNAPII) on protein-coding genes, but its underlying molecular mechanism is still poorly understood. Using in vitro transcription termination assays, we have found that RNAPII is prone to more effective termination by Rat1/Rai1 when its catalytic site is disrupted due to NTP misincorporation, implying that paused RNAPII, which is often found in vivo near termination sites, could adopt a similar configuration to Rat1/Rai1 and trigger termination. Intriguingly, yeast Rat1/Rai1 does not terminate Escherichia coli RNAP, implying that a specific interaction between Rat1/Rai1 and RNAPII may be required for termination. Furthermore, the efficiency of termination increases as the RNA transcript undergoing degradation by Rat1 gets longer, which suggests that Rat1 may generate a driving force for dissociating RNAPII from the template while degrading the nascent transcripts to catch up to the polymerase. These results indicate that multiple mechanistic features contribute to Rat1-mediated termination of RNAPII
URI
http://pr.ibs.re.kr/handle/8788114/1705
DOI
10.1093/nar/gkv133
ISSN
0305-1048
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
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