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Unraveling the mechanistic features of RNA polymerase II termination by the 5 '-3 ' exoribonuclease Rat1

DC Field Value Language
dc.contributor.authorJieun Park-
dc.contributor.authorMyungjin Kang-
dc.contributor.authorMinkyu Kim-
dc.date.available2015-07-10T02:38:53Z-
dc.date.created2015-05-18ko
dc.date.issued2015-03-
dc.identifier.issn0305-1048-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/1705-
dc.description.abstractWithin a complex with Rai1, the 5'-3' exoribonuclease Rat1 promotes termination of RNA polymerase II (RNAPII) on protein-coding genes, but its underlying molecular mechanism is still poorly understood. Using in vitro transcription termination assays, we have found that RNAPII is prone to more effective termination by Rat1/Rai1 when its catalytic site is disrupted due to NTP misincorporation, implying that paused RNAPII, which is often found in vivo near termination sites, could adopt a similar configuration to Rat1/Rai1 and trigger termination. Intriguingly, yeast Rat1/Rai1 does not terminate Escherichia coli RNAP, implying that a specific interaction between Rat1/Rai1 and RNAPII may be required for termination. Furthermore, the efficiency of termination increases as the RNA transcript undergoing degradation by Rat1 gets longer, which suggests that Rat1 may generate a driving force for dissociating RNAPII from the template while degrading the nascent transcripts to catch up to the polymerase. These results indicate that multiple mechanistic features contribute to Rat1-mediated termination of RNAPII-
dc.description.uri1-
dc.language영어-
dc.publisherOXFORD UNIV PRESS-
dc.titleUnraveling the mechanistic features of RNA polymerase II termination by the 5 '-3 ' exoribonuclease Rat1-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000352487100019-
dc.identifier.scopusid2-s2.0-84937597324-
dc.identifier.rimsid19549ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorJieun Park-
dc.contributor.affiliatedAuthorMyungjin Kang-
dc.contributor.affiliatedAuthorMinkyu Kim-
dc.identifier.doi10.1093/nar/gkv133-
dc.identifier.bibliographicCitationNUCLEIC ACIDS RESEARCH, v.43, no.5, pp.2625 - 2637-
dc.citation.titleNUCLEIC ACIDS RESEARCH-
dc.citation.volume43-
dc.citation.number5-
dc.citation.startPage2625-
dc.citation.endPage2637-
dc.date.scptcdate2018-10-01-
dc.description.wostc10-
dc.description.scptc11-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusPROMOTES TRANSCRIPTION TERMINATION-
dc.subject.keywordPlusSACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusBINDING PROTEIN-
dc.subject.keywordPlusELONGATION-
dc.subject.keywordPlusYEAST-
dc.subject.keywordPlusFIDELITY-
dc.subject.keywordPlusCLEAVAGE-
dc.subject.keywordPlusSITES-
dc.subject.keywordPlusDNA-
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
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