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Bioorthogonal imaging probe for STING protein in live cell and fixed tissue

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Title
Bioorthogonal imaging probe for STING protein in live cell and fixed tissue
Author(s)
Hyelim Lee; Ju-Young Park; Jubeen Yoon; Wonjin Park; Miso Kang; Jeehee Lee; Gyubin Park; Minseob Koh; Hyejin Kim; Chang Ho Sohn; Sanghee Lee; Eunha Kim
Publication Date
2024-08
Journal
Sensors and Actuators, B: Chemical, v.412
Publisher
Elsevier BV
Abstract
Stimulator of interferon genes (STING) is an endoplasmic reticulum membrane protein that plays a vital role in innate immunity. In this study, we report a new bioorthogonal chemical probe derived from a previously reported synthetic STING agonist by properly installing a trans-cyclooctene group without perturbation of the efficacy of the ligand. The probe is employed to investigate localization dynamics of STING in live cultured human cells and fixed mouse brain tissues. We showed that an optimized labeling strategy employing the probe and tetrazine-silicon rhodamine fluorophore conjugates in a stepwise manner enables the successful tracking of STING in live cells using fluorescence microscopy. Overall, our bioorthogonal approach using the probe will facilitate in-depth investigations of intricate mechanisms associated with STING. © 2024
URI
https://pr.ibs.re.kr/handle/8788114/15141
DOI
10.1016/j.snb.2024.135773
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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