BROWSE

Related Scientist

cmsd's photo.

cmsd
분자분광학및동력학연구단
more info

ITEM VIEW & DOWNLOAD

Second-harmonic generation microscopy with synthetic aperture and computational adaptive optics

Cited 0 time in webofscience Cited 0 time in scopus
57 Viewed 0 Downloaded
Title
Second-harmonic generation microscopy with synthetic aperture and computational adaptive optics
Author(s)
Jungho Moon; Sungsam Kang; Ye-Chan Cho; Jin Hee Hong; Shin, Dong-Jin; Gong, Su-Hyun; Yoon, Seok-Chan; Wonshik Choi
Publication Date
2024-01
Journal
Optica, v.11, no.1, pp.128 - 136
Publisher
OSA Publishing
Abstract
Second-harmonic generation (SHG) microscopy is a powerful label-free imaging tool widely used to visualize collagen and muscle in biological tissues. However, traditional laser-scanning SHG microscopy requiring voxel scanning is time-intensive. Wide-field SHG microscopy was designed to bypass this restriction, but its application to deep tissue imaging is limited due to vulnerability to scattering and sample-induced aberrations.We introduce synthetic aperture SHG (SA-SHG) microscopy to attenuate the effect of multiple scattering noises. Our SA-SHG method coherently integrates amplitude and phase maps of wide-fieldSHGfields taken for different illumination angles, thereby enhancing the signal-to-noise ratio.We also develop computational adaptive optics SHG (CAO-SHG) microscopy to computationally correct the sample-induced aberrations. Our algorithm optimizes SHG fields' aperture synthesis to identify aberration maps, enabling the restoration of diffraction-limited imaging. We successfully apply this approach to real biological samples, demonstrating its potential for high-resolution imaging in complex biological environments. © 2024 Optica Publishing Group.
URI
https://pr.ibs.re.kr/handle/8788114/14862
DOI
10.1364/OPTICA.505189
ISSN
2334-2536
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse