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Exosome Precipitation by Ionic Strength Modulation: ExoPRISM

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Title
Exosome Precipitation by Ionic Strength Modulation: ExoPRISM
Author(s)
Vijaya Sunkara; Juhee Park; Jiyun Han; Jonathan Sabaté del Río; Cho, Hyun-Ju; Oh, In-Jae; Yoon-Kyoung Cho
Publication Date
2023-11
Journal
ACS Applied Materials and Interfaces, v.15, no.49, pp.56807 - 56819
Publisher
American Chemical Society
Abstract
Extracellular vesicles (EVs) are emerging as crucial materials for precision theragnostic applications. However, current separation methods are time-consuming, costly, and not scalable and deliver limited yields or purity. Here, we present EV precipitation by ionic strength modulation (ExoPRISM), a simple, low-cost, user-friendly, and readily adaptable approach for separating EVs in high yields without compromising their biological functions. Adding an electrolyte solution to blood plasma in small increments generates the sequential precipitation of proteins and EVs, allowing for fractional separation of EVs using low-speed centrifugation. The coprecipitated electrolytes are easily washed away, and the entire EV separation and washing process takes less than an hour. This approach successfully separates EVs from a broad range of volumes and types of biological fluids, including culture medium, urine, plasma, and serum, showing promise as a robust tool for next-generation liquid biopsies and regenerative medicine. © 2023 The Authors. Published by American Chemical Society.
URI
https://pr.ibs.re.kr/handle/8788114/14424
DOI
10.1021/acsami.3c13527
ISSN
1944-8244
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
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