BROWSE

Related Scientist

cn's photo.

cn
나노의학연구단
more info

ITEM VIEW & DOWNLOAD

Simultaneous electrophysiology and optogenetic perturbation of the same neurons in chronically implanted animals using μLED silicon probes

Cited 0 time in webofscience Cited 0 time in scopus
206 Viewed 0 Downloaded
Title
Simultaneous electrophysiology and optogenetic perturbation of the same neurons in chronically implanted animals using μLED silicon probes
Author(s)
Nathaniel R. Kinsky; Mihály Vöröslakos; Jose Roberto Lopez Ruiz; Laurel Watkins de Jong; Nathan Slager; Sam McKenzie; Euisik Yoon; Kamran Diba
Publication Date
2023-12
Journal
STAR Protocols, v.4, no.4
Publisher
Cell Press
Abstract
Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneous in vivo electrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment. For complete details on the use and execution of this protocol, please refer to Watkins de Jong et al. (2023).1 © 2023 The Authors
URI
https://pr.ibs.re.kr/handle/8788114/14013
DOI
10.1016/j.xpro.2023.102570
ISSN
2666-1667
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse