BROWSE

Related Scientist

cgi's photo.

cgi
유전체항상성연구단
more info

ITEM VIEW & DOWNLOAD

A single-cell, time-resolved profiling of Xenopus mucociliary epithelium reveals nonhierarchical model of development

Cited 0 time in webofscience Cited 0 time in scopus
137 Viewed 0 Downloaded
Title
A single-cell, time-resolved profiling of Xenopus mucociliary epithelium reveals nonhierarchical model of development
Author(s)
Lee, Julie; Møller, Andreas Fønss; Chae, Shinhyeok; Bussek, Alexandra; Park, Tae Joo; Kim, Youni; Lee, Hyun-Shik; Pers, Tune H.; Taejoon Kwon; Sedzinski, Jakub; Natarajan, Kedar Nath
Publication Date
2023-04
Journal
Science advances, v.9, no.14
Publisher
NLM (Medline)
Abstract
The specialized cell types of the mucociliary epithelium (MCE) lining the respiratory tract enable continuous airway clearing, with its defects leading to chronic respiratory diseases. The molecular mechanisms driving cell fate acquisition and temporal specialization during mucociliary epithelial development remain largely unknown. Here, we profile the developing Xenopus MCE from pluripotent to mature stages by single-cell transcriptomics, identifying multipotent early epithelial progenitors that execute multilineage cues before specializing into late-stage ionocytes and goblet and basal cells. Combining in silico lineage inference, in situ hybridization, and single-cell multiplexed RNA imaging, we capture the initial bifurcation into early epithelial and multiciliated progenitors and chart cell type emergence and fate progression into specialized cell types. Comparative analysis of nine airway atlases reveals an evolutionary conserved transcriptional module in ciliated cells, whereas secretory and basal types execute distinct function-specific programs across vertebrates. We uncover a continuous nonhierarchical model of MCE development alongside a data resource for understanding respiratory biology.
URI
https://pr.ibs.re.kr/handle/8788114/13639
DOI
10.1126/sciadv.add5745
ISSN
2375-2548
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse