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DNA polymerase theta-mediated repair of high LET radiation-induced complex DNA double-strand breaks

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Title
DNA polymerase theta-mediated repair of high LET radiation-induced complex DNA double-strand breaks
Author(s)
Geunil Yi; Yubin Sung; Kim, Chanwoo; Jae Sun Ra; Hirakawa, Hirokazu; Kato, Takamitsu A.; Fujimori, Akira; Hajin Kim; Kei-ichi Takata
Publication Date
2023-03
Journal
NUCLEIC ACIDS RESEARCH, v.51, no.5, pp.2257 - 2269
Publisher
OXFORD UNIV PRESS
Abstract
DNA polymerase theta (POLQ) is a unique DNA polymerase that is able to perform microhomology-mediated end-joining as well as translesion synthesis (TLS) across an abasic (AP) site and thymine glycol (Tg). However, the biological significance of the TLS activity is currently unknown. Herein we provide evidence that the TLS activity of POLQ plays a critical role in repairing complex DNA double-strand breaks (DSBs) induced by high linear energy transfer (LET) radiation. Radiotherapy with high LET radiation such as carbon ions leads to more deleterious biological effects than corresponding doses of low LET radiation such as X-rays. High LET-induced DSBs are considered to be complex, carrying additional DNA damage such as AP site and Tg in close proximity to the DSB sites. However, it is not clearly understood how complex DSBs are processed in mammalian cells. We demonstrated that genetic disruption of POLQ results in an increase of chromatid breaks and enhanced cellular sensitivity following treatment with high LET radiation. At the biochemical level, POLQ was able to bypass an AP site and Tg during end-joining and was able to anneal two single-stranded DNA tails when DNA lesions were located outside the microhomology. This study offers evidence that POLQ is directly involved in the repair of complex DSBs.
URI
https://pr.ibs.re.kr/handle/8788114/13088
DOI
10.1093/nar/gkad076
ISSN
0305-1048
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
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