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SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
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- Title
- SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
- Author(s)
- Wu, S.-H.S.; Heetak Lee; Szép-Bakonyi, R.; Colozza, G.; Boese, A.; Gert, K.R.; Hallay, N.; Ji Hyun Lee; Kim, J.; Zhu, Y.; Linssen, M.M.; Pilat-Carotta, S.; Hohenstein, P.; Theussl, H.-C.; Pauli, A.; Bonkyoung Koo
- Publication Date
- 2022-12
- Journal
- Experimental and Molecular Medicine, v.54, no.12, pp.2188 - 2199
- Publisher
- Springer Nature
- Abstract
- The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging. © 2022, The Author(s).
- ISSN
- 1226-3613
- Appears in Collections:
- Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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